Allergen alters IL-2/αIL-2-based Treg expansion but not tolerance induction in an allergen-specific mouse model.

ABSTRACT:

Background

Regulatory T lymphocytes (Treg) play an important role in preventing allergic diseases. We characterized Treg expansion kinetics, marker profiles, and recirculation behavior in allergen-challenged mice, which had been pretreated with IL-2/αIL-2 complexes in the presence or absence of allergen. Moreover, the ability of induced Treg to control airway hyperreactivity and effector functions of lung T cells was determined.

Methods

Humanized TCR/HLA-transgenic allergy mice were treated in vivo with recombinant IL-2 complexed to the anti-IL-2 mAb JES6-1 in the presence or absence of mugwort pollen extract (MPE) on days 0-2. Afterward, they were intranasally challenged with MPE (days 13-15) followed by determination of airway hyperreactivity and lung T cell effector funct

SUBMITTER: Kohler C 

PROVIDER: S-EPMC7383865 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature