Process intensification for cytochrome P450 BM3-catalyzed oxy-functionalization of dodecanoic acid.

ABSTRACT: Selective oxy-functionalization of nonactivated C-H bonds is a long-standing "dream reaction" of organic synthesis for which chemical methodology is not well developed. Mono-oxygenase enzymes are promising catalysts for such oxy-functionalization to establish. Limitation on their applicability arises from low reaction output. Here, we showed an integrated approach of process engineering to the intensification of the cytochrome P450 BM3-catalyzed hydroxylation of dodecanoic acid (C12:0). Using P450 BM3 together with glucose dehydrogenase for regeneration of nicotinamide adenine dinucleotide phosphate (NADPH), we compared soluble and co-immobilized enzymes in O₂ -gassed and pH-controlled conversions at high final substrate concentrations (≥40mM). We identified the main engineering

SUBMITTER: Buergler MB 

PROVIDER: S-EPMC7384007 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature