Development and comparative study of a pat/bar real-time PCR assay for integrating the screening strategy of a GMO testing laboratory.

ABSTRACT: BACKGROUND:The number and variety of genetically modified organisms (GMOs) used globally for the production of food and feed, and potentially circulating in the European Union (EU), is constantly increasing. This implies an additional effort for the EU enforcement laboratories to optimize available resources, to contain costs and time. A well established approach for streamlining the analytical workflow is the introduction of a screening step, typically based on a smart set of real-time polymerase chain reaction (PCR) screening methods. The multiplexing strategy, allowing the detection of several screening elements simultaneously, is a further optimization of this step. RESULTS:In this study, we present the validation of a real-time PCR duplex assay for the pat and bar screening elements t

SUBMITTER: Verginelli D 

PROVIDER: S-EPMC7384061 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature