Comparison of five assays for DNA extraction from bacterial cells in human faecal samples.

ABSTRACT: AIM:To determine the most effective DNA extraction method for bacteria in faecal samples. MATERIALS AND RESULTS:This study assessed five commercial methods, that is, NucliSens easyMag, QIAamp DNA Stool Mini kit, PureLink Microbiome DNA purification kit, QIAamp PowerFecal DNA kit and RNeasy PowerMicrobiome kit, of which the latter has been optimized for DNA extraction. The DNA quantity and quality were determined using Nanodrop, Qubit and qPCR. The PowerMicrobiome kit recovered the highest DNA concentration, whereby this kit also recovered the highest gene copy number of Gram positives, Gram negatives and total bacteria. Furthermore, the PowerMicrobiome kit in combination with mechanical pre-treatment (bead beating) and with combined enzymatic and mechanical pre-treatment (proteinase K+muta

SUBMITTER: Gryp T 

PROVIDER: S-EPMC7384110 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature