Mitochondrial respiration variability and simulations in human skeletal muscle: The Gene SMART study.

ABSTRACT: Mitochondrial respiration using the oxygraph-2k respirometer (Oroboros) is widely used to estimate mitochondrial capacity in human skeletal muscle. Here, we measured mitochondrial respiration variability, in a relatively large sample, and for the first time, using statistical simulations, we provide the sample size required to detect meaningful respiration changes following lifestyle intervention. Muscle biopsies were taken from healthy, young men from the Gene SMART cohort, at multiple time points. We utilized samples for each measurement with two technical repeats using two respirometer chambers (n = 160 pairs of same muscle after removal of low-quality samples). We measured the Technical Error of measurement (TEM ) and the coefficient of variation (CV) for each mitochondrial complex. Th

SUBMITTER: Jacques M 

PROVIDER: S-EPMC7384122 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature