Synthesis of Galactosyl-Queuosine and Distribution of Hypermodified Q-Nucleosides in Mouse Tissues.

ABSTRACT: Queuosine (Q) is a hypermodified RNA nucleoside that is found in tRNA^His , tRNA^Asn , tRNA^Tyr , and tRNA^Asp . It is located at the wobble position of the tRNA anticodon loop, where it can interact with U as well as C bases located at the respective position of the corresponding mRNA codons. In tRNA^Tyr and tRNA^Asp of higher eukaryotes, including humans, the Q base is for yet unknown reasons further modified by the addition of a galactose and a mannose sugar, respectively. The reason for this additional modification, and how the sugar modification is orchestrated with Q formation and insertion, is unknown. Here, we report a total synthesis of the hypermodified nucleoside galactosyl-queuosine (galQ). The availability of the comp

SUBMITTER: Thumbs P 

PROVIDER: S-EPMC7384130 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature