Metabolic engineering of Escherichia coli for optimized biosynthesis of nicotinamide mononucleotide, a noncanonical redox cofactor.

ABSTRACT:

Background

Noncanonical redox cofactors are emerging as important tools in cell-free biosynthesis to increase the economic viability, to enable exquisite control, and to expand the range of chemistries accessible. However, these noncanonical redox cofactors need to be biologically synthesized to achieve full integration with renewable biomanufacturing processes.

Results

In this work, we engineered Escherichia coli cells to biosynthesize the noncanonical cofactor nicotinamide mononucleotide (NMN⁺), which has been efficiently used in cell-free biosynthesis. First, we developed a growth-based screening platform to identify effective NMN⁺ biosynthetic pathways in E. coli. Second, we explored various pathway combinations and host gene disruption to achieve a

SUBMITTER: Black WB 

PROVIDER: S-EPMC7384224 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature