Non-Arrhenius Reaction-Diffusion Kinetics for Protein Inactivation over a Large Temperature Range.

ABSTRACT: Understanding protein folding and unfolding has been a long-standing fundamental question and has important applications in manipulating protein activity in biological systems. Experimental investigations of protein unfolding have been predominately conducted by small temperature perturbations (e.g., temperature jump), while molecular simulations are limited to small time scales (microseconds) and high temperatures to observe unfolding. Thus, it remains unclear how fast a protein unfolds irreversibly and loses function (i.e., inactivation) across a large temperature range. In this work, using nanosecond pulsed heating of individual plasmonic nanoparticles to create precise localized heating, we examine the protein inactivation kinetics at extremely high temperatures. Connecting this with p

SUBMITTER: Sarkar D 

PROVIDER: S-EPMC7384293 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature