Streamlined downstream process for efficient and sustainable (Fab')₂ antivenom preparation.

ABSTRACT:

Background

Antivenoms are the only validated treatment against snakebite envenoming. Numerous drawbacks pertaining to their availability, safety and efficacy are becoming increasingly evident due to low sustainability of current productions. Technological innovation of procedures generating therapeutics of higher purity and better physicochemical characteristics at acceptable cost is necessary. The objective was to develop at laboratory scale a compact, feasible and economically viable platform for preparation of equine F(ab')₂ antivenom against Vipera ammodytes ammodytes venom and to support it with efficiency data, to enable estimation of the process cost-effectiveness.

Methods

The principle of simultaneous caprylic acid precipitation and pepsin digestion

SUBMITTER: Kurtovic T 

PROVIDER: S-EPMC7384442 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature