In Vivo Selection for Formate Dehydrogenases with High Efficiency and Specificity toward NADP.

ABSTRACT: The efficient regeneration of cofactors is vital for the establishment of biocatalytic processes. Formate is an ideal electron donor for cofactor regeneration due to its general availability, low reduction potential, and benign byproduct (CO₂). However, formate dehydrogenases (FDHs) are usually specific to NAD⁺, such that NADPH regeneration with formate is challenging. Previous studies reported naturally occurring FDHs or engineered FDHs that accept NADP⁺, but these enzymes show low kinetic efficiencies and specificities. Here, we harness the power of natural selection to engineer FDH variants to simultaneously optimize three properties: kinetic efficiency with NADP⁺, specificity toward NADP⁺, and affinity toward formate. By simultaneo

SUBMITTER: Calzadiaz-Ramirez L 

PROVIDER: S-EPMC7384739 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature