Modulating electrophysiology of motor neural networks via optogenetic stimulation during neurogenesis and synaptogenesis.

ABSTRACT: Control of electrical activity in neural circuits through network training is a grand challenge for biomedicine and engineering applications. Past efforts have not considered evoking long-term changes in firing patterns of in-vitro networks by introducing training regimens with respect to stages of neural development. Here, we used Channelrhodopsin-2 (ChR2) transfected mouse embryonic stem cell (mESC) derived motor neurons to explore short and long-term programming of neural networks by using optical stimulation implemented during neurogenesis and synaptogenesis. Not only did we see a subsequent increase of neurite extensions and synaptophysin clustering, but by using electrophysiological recording with micro electrode arrays (MEA) we also observed changes in signal frequency spectra, incr

SUBMITTER: Pagan-Diaz GJ 

PROVIDER: S-EPMC7385114 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature