Development of an SYBR Green I-based real-time PCR assay for the rapid detection of canine kobuvirus.

ABSTRACT: Canine kobuvirus (CaKoV) is a causative agent of gastroenteritis in dogs. Rapid detection of CaKoV is important for preventing and controlling this condition. In this study, an SYBR Green I-based quantitative real-time PCR assay was established for CaKoV detection. Specific primers targeting a highly conserved region of the CaKoV 3D gene were developed. After optimization, the method detected a minimum of 1 × 10¹ copies/μL with high specificity, stability, and repeatability. Moreover, the entire process only required approximately 1.5 h for completion. Our results were supported by those obtained for clinical samples, in which our developed method was successfully applied. The newly established real-time PCR is a rapid, sensitive, specific, and repeatable method for the quantitat

SUBMITTER: Wang Y 

PROVIDER: S-EPMC7385718 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature