Simulation-directed amplifiable nanoparticle enhanced quantitative scattering assay under low magnification dark field microscopy.

ABSTRACT: Nanoparticle-enhanced assays read by high-magnification dark-field microscopy require time-intensive analysis methods subject to selection bias, which can be resolved by using low magnification dark-field assays (LMDFA), at the cost of reduced sensitivity. We have simulated and experimentally validated a tunable linker-based signal amplification strategy yielding 6-fold enhanced LMDFA sensitivity.

SUBMITTER: Sun D 

PROVIDER: S-EPMC7386073 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature