Inhibition of miRNA‑135a‑5p ameliorates TGF‑β1‑induced human renal fibrosis by targeting SIRT1 in diabetic nephropathy.

ABSTRACT: miRNA‑135a‑5p upregulation has been identified in renal fibrosis in diabetic nephropathy (DN) with an incompletely known mechanism. Previous data showed that Sirtuin 1 (SIRT1) serves as a novel therapeutic target for DN and interact with the transforming growth factor‑β/mothers against decapentaplegic homolog (TGF‑β/Smad) signaling pathway. The aim of this study was to investigate the regulatory relationship between miR‑135a‑5p and SIRT1. The expression of miR‑135a‑5p and SIRT1 was detected using reverse transcription‑quantitative PCR and western blotting. The renal fibrosis and Smad3 signaling pathway were assessed by western blotting, by analyzing protein expression of collegen1A1, α‑smooth muscle actin (α‑SMA), fibronectin (FN), epithelial‑cadherin, Smad3 and phosphorylated Smad3 (p‑Sma

SUBMITTER: Zhang J 

PROVIDER: S-EPMC7387088 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature