Analysis of ex vivo expanded and activated clinical-grade human NK cells after cryopreservation.

ABSTRACT:

Background aims

Several methods to expand and activate (EA) NK cells ex vivo have been developed for the treatment of relapsed or refractory cancers. Infusion of fresh NK cells is generally preferred to the infusion of cryopreserved/thawed (C/T) NK cells because of concern that cryopreservation diminishes NK cell activity. However, there has been little head-to-head comparison of the functionality of fresh versus C/T NK cell products.

Methods

We evaluated activity of fresh and C/T EA NK cells generated by interleukin (IL)-15, IL-2 and CD137L expansion.

Results

Analysis of C/T NK cell products demonstrated decreased recovery of viable CD56⁺ cells, but the proportion of NK cells in the C/T EA NK cell product did not decrease compared with the fresh EA NK cell

SUBMITTER: Damodharan SN 

PROVIDER: S-EPMC7387178 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature