ABL1, Overexpressed in Hepatocellular Carcinomas, Regulates Expression of NOTCH1 and Promotes Development of Liver Tumors in Mice.

ABSTRACT:

Background & aims

We investigated whether ABL proto-oncogene 1, non-receptor tyrosine kinase (ABL1) is involved in development of hepatocellular carcinoma (HCC).

Methods

We analyzed clinical and gene expression data from The Cancer Genome Atlas. Albumin-Cre (Hep^WT) mice and mice with hepatocyte-specific disruption of Abl1 (Hep^Abl-/- mice) were given hydrodynamic injections of plasmids encoding the Sleeping Beauty transposase and transposons with the MET gene and a catenin β1 gene with an N-terminal truncation, which induces development of liver tumors. Some mice were then gavaged with the ABL1 inhibitor nilotinib or vehicle (control) daily for 4 weeks. We knocked down ABL1 with short hairpin RNAs in Hep3B and Huh7 HCC cells and analyzed their proliferat

SUBMITTER: Wang F 

PROVIDER: S-EPMC7387191 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature