Paraffin-embedding for large volume bio-tissue.

ABSTRACT: Acquiring ultrahigh-resolution three-dimensional images of large-volume tissues non-human primate tissues was an enormous challenge. Given the preservation of structure and excellent sectioning property, formalin-fixed paraffin-embedding method had an enormous potential for three-dimensional reconstruction of fine structures, based on the very thin histological sections and optical images. However, maintaining the structure uniformly in large-volume tissues was difficult during the complex processes. In this study, we presented a detailed protocol for the whole mouse, rat, rabbit brains, and even for the macaque hemisphere. The entire protocol took about 2-30 days to complete for a large sample, including fixation, dehydration, clearing, wax immersion and embedding. In addition, it could b

SUBMITTER: Zhanmu O 

PROVIDER: S-EPMC7387479 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature