Interactions of Monocytes, HIV, and ART Identified by an Innovative scRNAseq Pipeline: Pathways to Reservoirs and HIV-Associated Comorbidities.

ABSTRACT: HIV reservoirs persist despite successful antiretroviral therapy (ART) and are a major obstacle to the eradication and cure of HIV. The mature monocyte subset, CD14⁺CD16⁺, contributes to viral reservoirs and HIV-associated comorbidities. Only a subset of monocytes harbors HIV (HIV⁺), while the rest remain uninfected, exposed cells (HIV^exp). We developed an innovative single cell RNA sequencing (scRNAseq) pipeline that detects HIV and host transcripts simultaneously, enabling us to examine differences between HIV⁺ and HIV^exp mature monocytes. Using this, we characterized uninfected, HIV⁺, and HIV^exp primary human mature monocytes with and without ART. We showed that HIV⁺ mature monocytes do no

SUBMITTER: Leon-Rivera R 

PROVIDER: S-EPMC7387797 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature