Early transcriptome responses of the bovine midcycle corpus luteum to prostaglandin F2? includes cytokine signaling.

ABSTRACT: In ruminants, prostaglandin F2alpha (PGF2?)-mediated luteolysis is essential prior to estrous cycle resumption, and is a target for improving fertility. To deduce early PGF2?-provoked changes in the corpus luteum a short time-course (0.5-4 h) was performed on cows at midcycle. A microarray-determined transcriptome was established and examined by bioinformatic pathway analysis. Classic PGF2? effects were evident by changes in early response genes (FOS, JUN, ATF3) and prediction of active pathways (PKC, MAPK). Several cytokine transcripts were elevated and NF-?B and STAT activation were predicted by pathway analysis. Self-organizing map analysis grouped differentially expressed transcripts into ten mRNA expression patterns indicative of temporal signaling cascades. Comparison with two analogous datasets revealed a conserved group of 124 transcripts similarly altered by PGF2? treatment, which both, directly and indirectly, indicated cytokine activation. Elevated levels of cytokine transcripts after PGF2? and predicted activation of cytokine pathways implicate inflammatory reactions early in PGF2?-mediated luteolysis.

SUBMITTER: Talbott H 

PROVIDER: S-EPMC7388008 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature