Metabolic regulation of endothelial SK channels and human coronary microvascular function.

ABSTRACT:

Background

Diabetic (DM) inactivation of small conductance calcium-activated potassium (SK) channels contributes to coronary endothelial dysfunction. However, the mechanisms responsible for this down-regulation of endothelial SK channels are poorly understood. Thus, we hypothesized that the altered metabolic signaling in diabetes regulates endothelial SK channels and human coronary microvascular function.

Methods

Human atrial tissue, coronary arterioles and coronary artery endothelial cells (HCAECs) obtained from DM and non-diabetic (ND) patients (n = 12/group) undergoing cardiac surgery were used to analyze metabolic alterations, endothelial SK channel function, coronary microvascular reactivity and SK gene/protein expression/localization.

Results

The relaxation resp

SUBMITTER: Liu Y 

PROVIDER: S-EPMC7388214 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature