Microarray profiling and functional analysis of differentially expressed plasma exosomal circular RNAs in Graves' disease.

ABSTRACT:

Background

Circulating RNA (circRNA) regulates various bioactivities in cells. A better understanding of the exosomal circRNA can provide novel insights into the pathogenesis and treatment of Graves' disease (GD). We aimed to profile the differentially expressed circRNAs (DEcRs) in plasma exosomes of patients with GD and speculate and probe the functions of the DEcR by comprehensive bioinformatics analyses.

Methods

Serum exosomes were isolated from five primary GD patients and five healthy controls via ultracentrifugation. After verification with transmission electron microscopy, exosome samples were subjected to microarray profiling using human circRNA microarrays. Two up-regulated and two down-regulated DEcRs were selected for validation in plasma exosomes from 20 GD and 2

SUBMITTER: Sun Y 

PROVIDER: S-EPMC7388456 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature