Characterization and comparison of genomic profiles between primary cancer cell lines and parent atypical meningioma tumors.

ABSTRACT:

Background

Meningiomas are the second most common primary tumors of the central nervous system. However, there is a paucity of data on meningioma biology due to the lack of suitable preclinical in vitro and in vivo models. In this study, we report the establishment and characterization of patient-derived, spontaneously immortalized cancer cell lines derived from World Health Organization (WHO) grade I and atypical WHO grade II meningiomas.

Methods

We evaluated high-resolution 3T MRI neuroimaging findings in meningioma patients which were followed by histological analysis. RT-qPCR and immunostaining analyses were performed to determine the expression levels of meningioma-related factors. Additionally, flow cytometry and sorting assays were conducted to investigate and isolate

SUBMITTER: Kim E 

PROVIDER: S-EPMC7388534 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature