Intestinal PPARα Protects Against Colon Carcinogenesis via Regulation of Methyltransferases DNMT1 and PRMT6.

ABSTRACT:

Background & aims

Many genetic and environmental factors, including family history, dietary fat, and inflammation, increase risk for colon cancer development. Peroxisome proliferator-activated receptor alpha (PPARα) is a nuclear receptor that regulates systemic lipid homeostasis. We explored the role of intestinal PPARα in colon carcinogenesis.

Methods

Colon cancer was induced in mice with intestine-specific disruption of Ppara (Ppara^ΔIE), Ppara^fl/fl (control), and mice with disruption of Ppara that express human PPARA (human PPARA transgenic mice), by administration of azoxymethane with or without dextran sulfate sodium (DSS). Colons were collected from mice and analyzed by immunoblots, quantitative polymerase chain reaction, and histopathology. Liquid

SUBMITTER: Luo Y 

PROVIDER: S-EPMC7388731 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature