GtcA is required for LTA glycosylation in <i>Listeria monocytogenes</i> serovar 1/2a and <i>Bacillus subtilis</i>.

ABSTRACT: The cell wall polymers wall teichoic acid (WTA) and lipoteichoic acid (LTA) are often modified with glycosyl and D-alanine residues. Recent studies have shown that a three-component glycosylation system is used for the modification of LTA in several Gram-positive bacteria including Bacillus subtilis and Listeria monocytogenes. In the L. monocytogenes 1/2a strain 10403S, the cytoplasmic glycosyltransferase GtlA is thought to use UDP-galactose to produce the C₅₅-P-galactose lipid intermediate, which is transported across the membrane by an unknown flippase. Next, the galactose residue is transferred onto the LTA backbone on the outside of the cell by the glycosyltransferase GtlB. Here we show that GtcA is necessary for the glycosylation of LTA in L. monocyt

SUBMITTER: Rismondo J 

PROVIDER: S-EPMC7389260 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature