[Molecular epidemiology of norovirus in children with acute gastroenteritis in Tianjin, China].

ABSTRACT:

Objective

To investigate the molecular epidemiological characteristics of norovirus (NoV) among children with acute gastroenteritis in Tianjin in 2017.

Methods

A total of 758 stool specimens were collected from the children with acute gastroenteritis possibly caused by viral infection in Tianjin Children's Hospital between January and December, 2017. Quantitative real-time RT-PCR was used for primary screening of NoV, and conventional RT-PCR was used for gene amplification, sequencing and genotype identification of the VP1 region of capsid protein in positive specimens.

Results

Among the 758 specimens, 241 (31.8%) were found to have GII NoV. Sequencing of the VP1 region of capsid protein in positive specimens showed that among the 241 specimens with GII NoV, 69 (28.6%

SUBMITTER: Fang YL 

PROVIDER: S-EPMC7389835 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature