Essential role of Salmonella Enteritidis DNA adenine methylase in modulating inflammasome activation.

ABSTRACT:

Background

Salmonella Enteritidis (SE) is one of the major foodborne zoonotic pathogens of worldwide importance which can induce activation of NLRC4 and NLRP3 inflammasomes during infection. Given that the inflammasomes play an essential role in resisting bacterial infection, Salmonella has evolved various strategies to regulate activation of the inflammasome, most of which largely remain unclear.

Results

A transposon mutant library in SE strain C50336 was screened for the identification of the potential factors that regulate inflammasome activation. We found that T3SS-associated genes invC, prgH, and spaN were required for inflammasome activation in vitro. Interestingly, C50336 strains with deletion or overexpression of Dam were both defective in activation of caspase-1, se

SUBMITTER: Guo Y 

PROVIDER: S-EPMC7389876 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature