[Detection and analysis of bocavirus in hospitalized children with respiratory infection].

ABSTRACT:

Objective

To detect human bocavirus (HBoV) and investigate its genetic and evolutionary characteristics in children with acute respiratory infection in Tianjin, China.

Methods

A total of 1,259 samples of nasopharyngeal aspirates were collected from children with a confirmed diagnosis of acute respiratory infection between January and December, 2012. Viral nucleic acid was extracted, HBoV was detected by real-time quantitative PCR, and the gene segments of nucleocapsid protein of HBoV in positive samples were amplified by PCR. Several products were randomly selected and sequenced.The sequence obtained was compared with the known sequence of HBoV, and a phylogenetic analysis was performed. All the samples were examined to detect for other common respiratory tract viruses.

R

SUBMITTER: Wang W 

PROVIDER: S-EPMC7390088 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature