AlbaTraDIS: Comparative analysis of large datasets from parallel transposon mutagenesis experiments.

ABSTRACT: Bacteria need to survive in a wide range of environments. Currently, there is an incomplete understanding of the genetic basis for mechanisms underpinning survival in stressful conditions, such as the presence of anti-microbials. Transposon directed insertion-site sequencing (TraDIS) is a powerful tool to identify genes and networks which are involved in survival and fitness under a given condition by simultaneously assaying the fitness of millions of mutants, thereby relating genotype to phenotype and contributing to an understanding of bacterial cell biology. A recent refinement of this approach allows the roles of essential genes in conditional stress survival to be inferred by altering their expression. These advancements combined with the rapidly falling costs of sequencing now allows

SUBMITTER: Page AJ 

PROVIDER: S-EPMC7390408 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature