FGF2 modulates simultaneously the mode, the rate of division and the growth fraction in cultures of radial glia.

ABSTRACT: Radial glial progenitors in the mammalian developing neocortex have been shown to follow a deterministic differentiation program restricted to an asymmetric-only mode of division. This feature seems incompatible with their well-known ability to increase in number when cultured in vitro, driven by fibroblast growth factor 2 and other mitogenic signals. The changes in their differentiation dynamics that allow this transition from in vivo asymmetric-only division mode to an in vitro self-renewing culture have not been fully characterized. Here, we combine experiments of radial glia cultures with numerical models and a branching process theoretical formalism to show that fibroblast growth factor 2 has a triple effect by simultaneously increasing the growth fraction, promoting symmetric divisio

SUBMITTER: Ledesma-Terron M 

PROVIDER: S-EPMC7390635 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature