Characterization of regulatory T cell expansion for manufacturing cellular immunotherapies.

ABSTRACT: Regulatory T cells (T_regs) are critical mediators of peripheral immune tolerance. T_regs suppress immune activation against self-antigens and are the focus of cell-based therapies for autoimmune diseases. However, T_regs circulate at a very low frequency in blood, limiting the number of cells that can be isolated by leukapheresis. To effectively expand T_regsex vivo for cell therapy, we report the metabolic modulation of T cells using mono-(6-amino-6-deoxy)-β-cyclodextrin (βCD-NH₂) encapsulated rapamycin (Rapa). Encapsulating Rapa in β-cyclodextrin increased its aqueous solubility ∼154-fold and maintained bioactivity for at least 30 days. βCD-NH₂-Rapa complexes (CRCs) enriched the fraction of CD4⁺CD25⁺FoxP

SUBMITTER: McBride DA 

PROVIDER: S-EPMC7390695 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature