Developmental data for several human mitochondrial DNA (mtDNA) long amplification targets.

ABSTRACT: Candidate long mtDNA targets ∼300 bp in length were identified on the revised Cambridge mtDNA reference sequence using Primer Express software (Applied Biosystems) with modified default analysis settings. The primer and hydrolysis probe sequences for the resultant three (3) targets were queried in the Mitomap database [1] to avoid common single nucleotide polymorphisms (SNPs) which, if present in a sample, could reduce binding to template and therefore result in inefficient amplification. Primers and probes identified by Primer Express, some synthesized degenerate to mitigate the presence of certain SNPs, were utilized in a Fast Advanced Master Mix (Applied Biosystems) reaction which was amplified on a 7500 Real Time PCR System using HID Real Time PCR Software v1.2 (Applied Biosystems) to

SUBMITTER: Kavlick MF 

PROVIDER: S-EPMC7390771 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature