Genotyping-free parentage assignment using RAD-seq reads.

ABSTRACT: Parentage assignment is defined as the identification of the true parents of one focal offspring among a list of candidates and has been commonly used in zoological, ecological, and agricultural studies. Although likelihood-based parentage assignment is the preferred method in most cases, it requires genotyping a predefined set of DNA markers and providing their population allele frequencies. In the present study, we proposed an alternative method of parentage assignment that does not depend on genotype data and prior information of allele frequencies. Our method employs the restriction site-associated DNA sequencing (RAD-seq) reads for clustering into the overlapped RAD loci among the compared individuals, following which the likelihood ratio of parentage assignment could be directly calc

SUBMITTER: Chen SY 

PROVIDER: S-EPMC7391307 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature