The PI3K pathway impacts stem gene expression in a set of glioblastoma cell lines.

ABSTRACT:

Background

The PI3K pathway controls diverse cellular processes including growth, survival, metabolism, and apoptosis. Nuclear FOXO factors were observed in cancers that harbor constitutively active PI3K pathway output and stem signatures. FOXO1 and FOXO3 were previously published to induce stem genes such as OCT4 in embryonic stem cells. Here, we investigated FOXO-driven stem gene expression in U87MG glioblastoma cells.

Methods

PI3K-activated cancer cell lines were investigated for changes in gene expression, signal transduction, and clonogenicity under conditions with FOXO3 disruption or exogenous expression. The impact of PI3K pathway inhibition on stem gene expression was examined in a set of glioblastoma cell lines.

Results

We found that CRISPR-Cas9-mediated FOXO

SUBMITTER: Martinez E 

PROVIDER: S-EPMC7391469 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature