Analysis of exosomal circRNAs upon irradiation in pancreatic cancer cell repopulation.

ABSTRACT:

Background

Pancreatic cancer is one of the most malignant tumors. However, radiotherapy can lead to tumor recurrence, which is caused by the residual surviving cells repopulation stimulated by some molecular released from dying cells. Exosomes may mediate cell-cell communication and transfer kinds of signals from the dying cells to the surviving cells for stimulating tumor repopulation. Circular RNAs (circRNAs) may be one vital kind of exosomal cargos involving in modulating cancer cell repopulation.

Methods

Next generation sequencing (NGS) and bioinformatics were performed to analyze and annotate the expression and function of exosome-derived circRNAs in pancreatic cancer cells after radiation. Four circRNAs were chosen for qRT-PCR analysis to validate the sequencing result

SUBMITTER: Chen YY 

PROVIDER: S-EPMC7391519 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature