Compared with conventional PCR assay, qPCR assay greatly improves the detection efficiency of predation.

ABSTRACT: Studies of predation can contribute greatly to understanding predator-prey relationships and can also provide integral knowledge concerning food webs and multi-trophic level interactions. Both conventional polymerase chain reaction (cPCR) and quantitative PCR (qPCR) have been employed to detect predation in the field because of their sensitivity and reproducibility. However, to date, few studies have been used to comprehensively demonstrate which method is more sensitive and reproducible in studies of predation. We used a Drosophila melanogaster-specific DNA fragment (99 bp) to construct a tenfold gradient dilution of standards. Additionally, we obtained DNA samples from Pardosa pseudoannulata individuals that fed on D. melanogaster at various time since feeding. Final

SUBMITTER: Yang TB 

PROVIDER: S-EPMC7391540 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature