Rapid detection of Babesia motasi responsible for human babesiosis by cross-priming amplification combined with a vertical flow.

ABSTRACT:

Background

Babesia motasi is known as an etiological agent of human and ovine babesiosis. Diagnosis of babesiosis is traditionally performed by microscopy, examining Giemsa-stained thin peripheral blood smears. Rapid detection and accurate identification of species are desirable for clinical care and epidemiological studies.

Methods

An easy to operate molecular method, which requires less capital equipment and incorporates cross-priming amplification combined with a vertical flow (CPA-VF) visualization strip for rapid detection and identification of B. motasi.

Results

The CPA-VF targets the 18S rRNA gene and has a detection limit of 50 fg per reaction; no cross reaction was observed with other piroplasms infective to sheep or Babesia infective to humans. CPA-VF and re

SUBMITTER: Wang J 

PROVIDER: S-EPMC7391542 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature