Constructing a novel expression system by specific activation of amylase expression pathway in Penicillium.

ABSTRACT:

Background

Filamentous fungi have long been used as hosts for the production of proteins, enzymes and valuable products in various biotechnological applications. However, recombinant proteins are expressed with highly secreted host proteins when stronger promoters are used under inducing conditions. In addition, the efficiency of target protein expression can be limited by the application of constitutive promoters in recently developed filamentous fungal expression systems.

Results

In this study, a novel expression system was constructed by using a Penicillium oxalium strain that has powerful protein secretion capability. The secretory background of the host was reduced by knocking out the Amy13A protein and utilizing the starch as a carbon source. The strong promoter amy15A

SUBMITTER: Pi C 

PROVIDER: S-EPMC7391575 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature