Developmental GABA polarity switch and neuronal plasticity in Bioengineered Neuronal Organoids.

ABSTRACT: Brain organoids are promising tools for disease modeling and drug development. For proper neuronal network formation excitatory and inhibitory neurons as well as glia need to co-develop. Here, we report the directed self-organization of human induced pluripotent stem cells in a collagen hydrogel towards a highly interconnected neuronal network at a macroscale tissue format. Bioengineered Neuronal Organoids (BENOs) comprise interconnected excitatory and inhibitory neurons with supportive astrocytes and oligodendrocytes. Giant depolarizing potential (GDP)-like events observed in early BENO cultures mimic early network activity of the fetal brain. The observed GABA polarity switch and reduced GDPs in >40 day BENO indicate progressive neuronal network maturation. BENOs demonstrate expedited co

SUBMITTER: Zafeiriou MP 

PROVIDER: S-EPMC7391775 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature