MCherry fusions enable the subcellular localization of periplasmic and cytoplasmic proteins in Xanthomonas sp.

ABSTRACT: Fluorescent markers are a powerful tool and have been widely applied in biology for different purposes. The genome sequence of Xanthomonas citri subsp. citri (X. citri) revealed that approximately 30% of the genes encoded hypothetical proteins, some of which could play an important role in the success of plant-pathogen interaction and disease triggering. Therefore, revealing their functions is an important strategy to understand the bacterium pathways and mechanisms involved in plant-host interaction. The elucidation of protein function is not a trivial task, but the identification of the subcellular localization of a protein is key to understanding its function. We have constructed an integrative vector, pMAJIIc, under the control of the arabinose promoter, which allows the inducible expr

SUBMITTER: Pena MM 

PROVIDER: S-EPMC7392301 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature