Robust, 3-Dimensional Visualization of Human Colon Enteric Nervous System Without Tissue Sectioning.

ABSTRACT:

Background & aims

Small, 2-dimensional sections routinely used for human pathology analysis provide limited information about bowel innervation. We developed a technique to image human enteric nervous system (ENS) and other intramural cells in 3 dimensions.

Methods

Using mouse and human colon tissues, we developed a method that combines tissue clearing, immunohistochemistry, confocal microscopy, and quantitative analysis of full-thickness bowel without sectioning to quantify ENS and other intramural cells in 3 dimensions.

Results

We provided 280 adult human colon confocal Z-stacks from persons without known bowel motility disorders. Most of our images were of myenteric ganglia, captured using a 20× objective lens. Full-thickness colon images, viewed with a 10× objecti

SUBMITTER: Graham KD 

PROVIDER: S-EPMC7392351 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature