Identification of an α-MoRF in the Intrinsically Disordered Region of the Escargot Transcription Factor.

ABSTRACT: Molecular recognition features (MoRFs) are common in intrinsically disordered proteins (IDPs) and intrinsically disordered regions (IDRs). MoRFs are in constant order-disorder structural transitions and adopt well-defined structures once they are bound to their targets. Here, we study Escargot (Esg), a transcription factor in Drosophila melanogaster that regulates multiple cellular functions, and consists of a disordered N-terminal domain and a group of zinc fingers at its C-terminal domain. We analyzed the N-terminal domain of Esg with disorder predictors and identified a region of 45 amino acids with high probability to form ordered structures, which we named S2. Through 54 μs of molecular dynamics (MD) simulations using CHARMM36 and implicit solvent (generalized Born/surface area

SUBMITTER: Hernandez-Segura T 

PROVIDER: S-EPMC7392517 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature