Peroxiredoxin promotes longevity and H₂O₂-resistance in yeast through redox-modulation of protein kinase A.

ABSTRACT: Peroxiredoxins are H₂O₂ scavenging enzymes that also carry out H₂O₂ signaling and chaperone functions. In yeast, the major cytosolic peroxiredoxin, Tsa1 is required for both promoting resistance to H₂O₂ and extending lifespan upon caloric restriction. We show here that Tsa1 effects both these functions not by scavenging H₂O₂, but by repressing the nutrient signaling Ras-cAMP-PKA pathway at the level of the protein kinase A (PKA) enzyme. Tsa1 stimulates sulfenylation of cysteines in the PKA catalytic subunit by H₂O₂ and a significant proportion of the catalytic subunits are glutathionylated on two cysteine residues. Redox modification of the conserved Cys243 inhibits the phosphoryla

SUBMITTER: Roger F 

PROVIDER: S-EPMC7392609 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature