Completion of draft bacterial genomes by long-read sequencing of synthetic genomic pools.

ABSTRACT:

Background

Illumina technology currently dominates bacterial genomics due to its high read accuracy and low sequencing cost. However, the incompleteness of draft genomes generated by Illumina reads limits their application in comprehensive genomics analyses. Alternatively, hybrid assembly using both Illumina short reads and long reads generated by single molecule sequencing technologies can enable assembly of complete bacterial genomes, yet the high per-genome cost of long-read sequencing limits the widespread use of this approach in bacterial genomics. Here we developed a protocol for hybrid assembly of complete bacterial genomes using miniaturized multiplexed Illumina sequencing and non-barcoded PacBio sequencing of a synthetic genomic pool (SGP), thus significantly decreasing th

SUBMITTER: Derakhshani H 

PROVIDER: S-EPMC7392658 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature