The length of ribosomal binding site spacer sequence controls the production yield for intracellular and secreted proteins by Bacillus subtilis.

ABSTRACT:

Background

Bacillus subtilis is widely used for the industrial production of recombinant proteins, mainly due to its high secretion capacity, but higher production yields can be achieved only if bottlenecks are removed. To this end, a crucial process is translation initiation which takes place at the ribosome binding site enclosing the Shine Dalgarno sequence, the start codon of the target gene and a short spacer sequence in between. Here, we have studied the effects of varying spacer sequence lengths in vivo on the production yield of different intra- and extracellular proteins.

Results

The shuttle vector pBSMul1 containing the strong constitutive promoter P_HpaII and the optimal Shine Dalgarno sequence TAAGGAGG was used as a template to construct a series of vect

SUBMITTER: Volkenborn K 

PROVIDER: S-EPMC7392706 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature