Three-dimensional bright-field microscopy with isotropic resolution based on multi-view acquisition and image fusion reconstruction.

ABSTRACT: Optical Projection Tomography (OPT) is a powerful three-dimensional imaging technique used for the observation of millimeter-scaled biological samples, compatible with bright-field and fluorescence contrast. OPT is affected by spatially variant artifacts caused by the fact that light diffraction is not taken into account by the straight-light propagation models used for reconstruction. These artifacts hinder high-resolution imaging with OPT. In this work we show that, by using a multiview imaging approach, a 3D reconstruction of the bright-field contrast can be obtained without the diffraction artifacts typical of OPT, drastically reducing the amount of acquired data, compared to previously reported approaches. The method, purely based on bright-field contrast of the unstained sample, prov

SUBMITTER: Calisesi G 

PROVIDER: S-EPMC7392767 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature