Canalicular system reorganization during mouse platelet activation as revealed by 3D ultrastructural analysis.

ABSTRACT: The canalicular system (CS) has been defined as: 1) an inward, invaginated membrane connector that supports entry into and exit from the platelet; 2) a static structure stable during platelet isolation; and 3) the major source of plasma membrane (PM) for surface area expansion during activation. Recent analysis from STEM tomography and serial block face electron microscopy has challenged the relative importance of CS as the route for granule secretion. Here, We used 3D ultrastructural imaging to reexamine the CS in mouse platelets by generating high-resolution 3D reconstructions to test assumptions 2 and 3. Qualitative and quantitative analysis of whole platelet reconstructions, obtained from immediately fixed or washed platelets fixed post-washing, indicated that CS,

SUBMITTER: Pokrovskaya ID 

PROVIDER: S-EPMC7392809 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature