Generation of Scalable Hepatic Micro-Tissues as a Platform for Toxicological Studies.

ABSTRACT:

Background

Currently, there is an urgent need for scalable and reliable in vitro models to assess the effects of therapeutic entities on the human liver. Hepatoma cell lines, including Huh-7, show weakly resemblance to human hepatocytes, limiting their significance in toxicity studies. Co-culture of hepatic cells with non-parenchymal cells, and the presence of extracellular matrix have been shown to influence the biological behavior of hepatocytes. The aim of this study was to generate the scalable and functional hepatic micro-tissues (HMTs).

Methods

The size-controllable HMTs were generated through co-culturing of Huh-7 cells by mesenchymal stem cells and human umbilical vein endothelial cells in a composite hydrogel of liver-derived extracellular matrix and alginate, using

SUBMITTER: Darakhshan S 

PROVIDER: S-EPMC7392990 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature