Open-channel blocking action of volatile anaesthetics desflurane and sevoflurane on human voltage-gated K_v 1.5 channel.

ABSTRACT:

Background and purpose

Volatile anaesthetics have been shown to differentially modulate mammalian Shaker-related voltage-gated potassium (K_v 1.x) channels. This study was designed to investigate molecular and cellular mechanisms underlying the modulatory effects of desflurane or sevoflurane on human K_v 1.5 (hK_v 1.5) channels.

Experimental approach

Thirteen single-point mutations were constructed within pore domain of hK_v 1.5 channel using site-directed mutagenesis. The effects of desflurane or sevoflurane on heterologously expressed wild-type and mutant hK_v 1.5 channels were examined by whole-cell patch-clamp technique. A computer simulation was conducted to predict the docking pose of desflurane or sevoflurane within hK

SUBMITTER: Fukushima Y 

PROVIDER: S-EPMC7393063 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature