Binding of S100A6 to actin and the actin-tropomyosin complex.

ABSTRACT: S100A6 is a low molecular weight Ca²⁺-binding protein belonging to the S100 family. Many reports indicate that in the cell S100A6 has an influence on the organization of actin filaments, but so far no direct interaction between S100A6 and actin has been shown. In the present study we investigated binding of S100A6 to actin and the actin-tropomyosin complex. The analyses were performed on G- and F-actin and two tropomyosin isoforms-Tpm1.6 and Tpm1.8. Using purified proteins and a variety of biochemical approaches we have shown that, in a Ca²⁺-bound form, S100A6 directly interacts with G- and F-actin and with tropomyosin, preferentially with isoform Tpm1.8. S100A6 and tropomyosin bind to the same population of filaments and the presence of tropomyosin on the microfilame

SUBMITTER: Jurewicz E 

PROVIDER: S-EPMC7393103 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature