Fluorescent bead-based serological detection of Toxoplasma gondii infection in chickens.

ABSTRACT:

Background

Free-ranging chickens are often infected with Toxoplasma gondii and seroconvert upon infection. This indicates environmental contamination with T. gondii.

Methods

Here, we established a bead-based multiplex assay (BBMA) using the Luminex technology for the detection of T. gondii infections in chickens. Recombinant biotinylated T. gondii surface antigen 1 (TgSAG1_bio) bound to streptavidin-conjugated magnetic Luminex beads served as antigen. Serum antibodies were detected by a fluorophore-coupled secondary antibody. Beads of differing color codes were conjugated with anti-chicken IgY or chicken serum albumin and served for each sample as an internal positive or negative control, respectively. The assay was validated with sera from experimentally and natur

SUBMITTER: Fabian BT 

PROVIDER: S-EPMC7393333 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature